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KMID : 0922220020020020081
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Journal of the Korean Musculoskeletal Transplantation Society
2002 Volume.2 No. 2 p.81 ~ p.91
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Sterilization of Human Tissues for Allo-transplantation
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Han Dong-Wook
Lee Dong-Hee
Shin Kyoo-Ho
Park Jong-Chul
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Abstract
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The use of allografts has been used in clinical practice for many years for the treatment of orthopaedic or cardiovascular disorders. There is always a risk of disease transmission with the use of allograft although it appears to be diminishing through the rigorous screening of donors, and improved harvesting and banking techniques. Whatever method is used, the graft cannot be guaranteed sterile and some centers use methods such as irradiation or ethylene oxide treatment for secondary decontamination of the grafts. These forms of sterilization can influence the biological and/or biomechanical characteristics of the allografts. Another method of reducing the risk of infection is to minimize contamination during the harvesting of the allograft in operating theaters. This contamination, through contact of the graft with the operating room air or with non-sterile surfaces, is also greater from cadaveric donors at the end of multiorgan harvest, than from living donors. Some experimental protocols recommend the rinsing of contaminated grafts with antiseptic or antibiotic solutions. Since antibiotic disinfection procedures were developed to treat heart valve allografts for tissue banking in 1969, several different combinations of antibiotics have been used to treat fresh and/or cryopreserved heart valves. However, antibiotic toxicity can be a problem in situations where cellular viability of the tissues is considered important. In the last part, development of antibiotics with modified composition to improve cellular viability and its practical applications to cryopreserved vascular tissues were described on the basis of our previous studies.
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KEYWORD
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Allografts, Sterilization, Contamination, Antibiotics, Cryopreservation, Cellular Viability
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